蛋白抽提試劑盒
亞細(xì)胞蛋白質(zhì)組抽提試劑盒
· 獲得4個(gè)組分:胞漿蛋白、膜蛋白、核蛋白及細(xì)胞骨架蛋白
· 無需超高速離心,也無需再較高溫度下孵育樣品
· 蛋白以天然活性狀態(tài)被富集
· 成分完整,包括抽提緩沖液、蛋白酶抑制劑、Benzonase核酸酶等
· 每個(gè)試劑盒可用于20次貼壁或懸浮細(xì)胞處理
蛋白抽提與樣品制備 | 貨號(hào) | 產(chǎn)品名稱及描述 | 規(guī)格 |
71456-3 | BugBuster Master Mix | 100ml |
71009-3 | CytoBuster Protein Extraction Reagent | 50ml |
20-188 | Ripa Lysis Buffer | 10*100ml |
71186-3 | YeastBuster Protein Extraction Reagent | 100ml |
70664-3 | Benzonase Purity>99% | 10ku |
539790-1kit | ProteoExtract Subcellular Proteome Extraction Kit | 20rxn |
71772-3 | ProteoExtract Transmembrane Protein Extraction Kit | 40rxn |
17-10195 | ProteoExtract Cytoskeleton Enrichment and Isolation Kit | 1kit |
71183 | NucBuster Protein Extraction Kit | 100rxn |
539134-1ML | Protease Inhibitor Cocktail Set III | 1ml |
539134-1SET | Protease Inhibitor Cocktail Set III | 1set |
71296-3 | Phosphosafe Extraction Reagent | 25ml |
524625-1SET | Phosphatase Inhibitor Cocktail Set II | 1set |
免疫沉淀產(chǎn)品——更快的免疫沉淀和蛋白質(zhì)純化
· 磁珠表面光滑,大幅度降低雜蛋白背景;
· 結(jié)果重復(fù)性好:磁力架上磁珠吸到管壁,能盡量出去液體而不吸走樣品;
· 更快速方便:無需離心,節(jié)省時(shí)間;
· 更高效:可拆卸的磁力架設(shè)計(jì),方便多個(gè)磁珠/樣品再架子上直接進(jìn)行震蕩混勻。
免疫沉淀 | 貨號(hào) | 產(chǎn)品名稱及描述 | 規(guī)格 |
LSKMAGAG02 | PureProteome Protein A/GMixMag Beads | 2*1ml |
LSKMAGAG10 | PureProteome Protein A/GMixMag Beads | 10ml |
LSKMAGS08 | PureProteome Magnetic Stand,8 well | 1 |
LSKMAGS15 | PureProteome Magnetic Stand,15ml | 1 |
16-125 | Protein A-Agarose | 10ml |
16-156 | Protein A Agarose Fast Flow | 10ml |
16-266 | Protein G Agarose Fast Flow | 10ml |
超濾濃縮產(chǎn)品
Amicon 超濾杯可進(jìn)行大體積樣品(50-400mL)大分子溶質(zhì)分離:
· 組裝、拆卸以及開關(guān)簡(jiǎn)便
· 整體安全性高:內(nèi)螺紋固定-內(nèi)置壓力鎖/釋放閥設(shè)計(jì),不再需要外部保護(hù)框架,不僅拆裝更加安全,也更便于確定超濾杯是否被正確地組裝
· 杜絕滲漏
· 攪拌棒設(shè)計(jì)更為合理、安全,減少對(duì)膜的損壞,膜片壽命更長
Amicon 超濾杯 |
容積 | 膜片直徑 | 目錄號(hào) |
50mL | 44.5mm | UFSC05001 |
200mL | 63.5mm | UFSC20001 |
400mL | 76mm | UFSC40001 |
微濾膜片 Durapore PVDF膜(25片/盒) |
膜孔徑 | 膜片直徑(mm) | 目錄號(hào) |
0.1 | 63.5 | WLP06225 |
76 | WLP07625 |
0.22 | 63.5 | GVWP06225 |
76 | GVWP07625 |
0.45 | 63.5 | HVLP06225 |
76 | HVLP07625 |
超濾膜片(10片/盒) |
膜材質(zhì) | NMWL(K) | 44.5mm | 63.5mm | 76mm |
Ultracel 再生纖維素膜 | 1 | PLAC04310 | PLAC06210 | PLAC07610 |
3 | PLBC04310 | PLBC06210 | PLBC07610 |
5 | PLCC04310 | PLCC06210 | PLCC07610 |
10 | PLGC04310 | PLGC06210 | PLGC07610 |
30 | PLTK04310 | PLTK06210 | PLTK07610 |
100 | PLHK04310 | PLHK06210 | PLHK07610 |
Biomax PES膜 | 5 | PBCC04310 | PBCC06210 | PBCC07610 |
10 | PBGC04310 | PBGC06210 | PBGC07610 |
30 | PBTK04310 | PBTK06210 | PBTK07610 |
50 | PBQK04310 | PBQK06210 | PBQK07610 |
100 | PBHK04310 | PBHK06210 | PBHK07610 |
300 | PBMK04310 | PBMK06210 | PBMK07610 |
500 | PBVK04310 | PBVK06210 | PBVK07610 |
超濾管
明確的死體積——避免樣品離干,獲得相對(duì)穩(wěn)定濃縮倍數(shù)
反轉(zhuǎn)離心——蛋白回收率提高,減少吸取時(shí)的操作失誤
雙豎膜——操作更快,減少膜堵塞
再生纖維素膜——低蛋白吸附,獨(dú)有的熱封工藝,避免膠水造成樣品污染
|
| Amicon Ultra-0.5 | Amicon Ultra-2 | Amicon Ultra-4 | Amicon Ultra-15 |
| 最大上樣體積(mL) | 0.5 | 2 | 4 | 15 |
| 濃縮產(chǎn)物終體積(uL) | 15-20 | 15-70 | 30-70 | 150-300 |
截留分子量 | 包裝數(shù) | 目錄號(hào) |
3kDa | 8 | UFC500308 |
| UFC800308 | UFC900308 |
| 24 | UFC500324 | UFC200324 | UFC800324 | UFC900324 |
| 96 | UFC500396 |
| UFC800396 | UFC900396 |
10kDa | 8 | UFC501008 |
| UFC801008 | UFC901008 |
| 24 | UFC501024 | UFC201024 | UFC801024 | UFC901024 |
| 96 | UFC501096 |
| UFC801096 | UFC901096 |
30kDa | 8 | UFC503008 |
| UFC803008 | UFC903008 |
| 24 | UFC503024 | UFC203024 | UFC803024 | UFC903024 |
| 96 | UFC503096 |
| UFC803096 | UFC903096 |
50kDa | 8 | UFC505008 |
| UFC805008 | UFC905008 |
| 24 | UFC505024 | UFC205024 | UFC805024 | UFC905024 |
| 96 | UFC505096 |
| UFC805096 | UFC905096 |
100kDa | 8 | UFC510008 |
| UFC810008 | UFC910008 |
| 24 | UFC510024 | UFC210024 | UFC810024 | UFC910024 |
| 96 | UFC510096 |
| UFC810096 | UFC910096 |
更多產(chǎn)品和介紹,請(qǐng)參考默克生命科學(xué)蛋白樣品制備官方網(wǎng)址。